Pages tagged with technique

Artificial chromosomes

Artificial chromosomes are advanced vectors, they have inserted elements to emulate chromosomes in different model organisms. Since the chromosomes...

Blotting

Blotting is the act of transferring a sample, via a membrane, from one place to another. The transfer can be accomplished either with an electric...

Cloning vectors

Cloning vectors are used for the purpose of multiplying a gene in a host cell. For the vector to be multiplied it needs to be transcribed within the...

Constitutive expression

Constitutive expression is the ability for a vector to constantly produce the inserted protein. The feature is dependent on the promoter controlling...

Cosmids

Plasmid containing cos sequences which are cohesive ends that are needed for packing the DNA into phage heads from phage lambda. The cosmids can...

Expression vectors

Expression vectors are used for the purpose of multiplying a specific protein in a host cell. The vector needs to contain the element that enable...

Inducible expression

Inducible expression is a feature of a vector where the expression of the insert is induced by an agent that is added at an appropriate time of the...

Plasmids

A plasmid is circular extra-chromosomal DNA found in most bacteria, but do also exist in eukaryots such as S. cerevisiae. Plasmids usually contain...

SDS PAGE

SDS PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) is the process of denaturing and cover proteins in a sample with negative...

Shuttle vectors

Shuttle vectors enable expression of genes in different organisms. Since gene recombination is easier in some organisms it is advantageous to be able...

Vectors

A vector is a vehicle in which a gene from one organisms can be transferred into a unicellular organism or a cell form a multicellular organism. By...

Western blot

Transfer of protein samples from a SDS-PAGE, where the samples have been denatured, streched and covede in the negative molecule SDS.

Viral vectors

The viral vectors is circular viral DNA. * genetic marker - for confirming integration into the host genome