Pages tagged with DNA

Artificial chromosomes

Artificial chromosomes are advanced vectors, they have inserted elements to emulate chromosomes in different model organisms. Since the chromosomes...

Cloning vectors

Cloning vectors are used for the purpose of multiplying a gene in a host cell. For the vector to be multiplied it needs to be transcribed within the...

Constitutive expression

Constitutive expression is the ability for a vector to constantly produce the inserted protein. The feature is dependent on the promoter controlling...

Cosmids

Plasmid containing cos sequences which are cohesive ends that are needed for packing the DNA into phage heads from phage lambda. The cosmids can...

DNA damage

There are many processes that can damage DNA: 1. Exogenous proceses, like radiation and chemicals 2. Endogenous processed, like a mutation...

DNA polymerase eta (DNA polĪ·)

DNA polymerase eta (pol eta) is one of few DNA polymerases that can bypass damage in the DNA during replication. This decreases the risk of greater...

DNA repair

Is preformed through a number of mechanisms that maintain the DNA intact and removes damage caused by exogenic or endogenic processes.

DNA replication

The process through which DNA is duplicated into two identical helices just before cell division.

DNA replication fork

The DNA replication fork is a complex of proteins that slide along the DNA upon replication and preforms the actual replication, i.e, analyses each...

Expression vectors

Expression vectors are used for the purpose of multiplying a specific protein in a host cell. The vector needs to contain the element that enable...

Inducible expression

Inducible expression is a feature of a vector where the expression of the insert is induced by an agent that is added at an appropriate time of the...

M-phase (Mitotc-phase)

Rapidly growing human cells go through M-phase in ~ 30 min

Plasmids

A plasmid is circular extra-chromosomal DNA found in most bacteria, but do also exist in eukaryots such as S. cerevisiae. Plasmids usually contain...

S-phase (Synthesis-phase)

In S-phase the genome is duplicated in a process called DNA replication. The DNA replication is preformed by a large protein complex collectively...

Vectors

A vector is a vehicle in which a gene from one organisms can be transferred into a unicellular organism or a cell form a multicellular organism. By...

Viral vectors

The viral vectors is circular viral DNA. * genetic marker - for confirming integration into the host genome

Shuttle vectors

Shuttle vectors enable expression of genes in different organisms. Since gene recombination is easier in some organisms it is advantageous to be able...

DNA polymerases

DNA polymerases are responsible for bypassing damage in the DNA during replication. If the damage is not bypassed the group of proteins driving the...